Author/Editor		Eržen, I; Janaček, J; Kubinova, L
Title		Characterisation of the capillary network in skeletal muscles from 3D data- a review
Type		članek
Source		Physiol Res
Vol. and No.		Letnik 60, št. 1
Publication year		2010
Volume		str. 1-13
Language		eng
Abstract		In this review paper we present immunohistochemical methods for visualisation of capillaries and muscle fibres in thick muscle sections. Special attention is paid to the procedures that preserve good morphology. Applying confocal microscopy and virtual 3D stereological grids, or tracing of capillaries in virtual reality, length of capillaries within a muscle volume or length of capillaries adjacent to a muscle fibre per fibre length, fibre surface area or fibre volume can be evaluated by an unbiased approach and 3D models of capillaries and muscle fibres can be produced. Comparison of the developed methods with counting capillary profiles from 2D sections is discussed and the reader is warned that counting capillary profiles from 2D sections can underestimate the capillary length by as much as 75 percent. Application of the described 3D methodology is illustrated by the anatomical remodelling of capillarity during acute denervation and early reinnervation in the rat soleus and extensor digitorum longus muscles.
Descriptors		MUSCLE, SKELETAL CAPILLARIES MUSCLE FIBERS IMMUNOHISTOCHEMISTRY MICROSCOPY, CONFOCAL IMAGE PROCESSING, COMPUTER-ASSISTED MUSCLE DENERVATION RATS, WISTAR