Author/Editor		Kapraali, MW; Johansson, O; Uribe, A
Title		Stereological estimation of the total volume of duodenal mucosa and of endocrine cells in rats treated with prostaglandin E2
Type		članek
Source		Acta Stereol
Vol. and No.		Letnik 13, št. 1
Publication year		1994
Volume		str. 27-30
Language		eng
Abstract		Stereological methods were applied on histological sections of 2 cm rat duodenum to determine the total volume of the mucosa, the total surface area of small intestinal epithelial Iining and the total volume of endocrine cells. The effects of different oral doses of prostaglandin E2 and a methyl analogue on the parameters above were estimated following 4 weeks of treatment. The total volume of serotoninimmunoreactive cells was increased by prostaglandins E2, whereas the analogue reduced the total volume of somatostatin-immunoreactive cells compared to controls (p less th. 0.05). The total mucosal volume and the total surface area of small intestinal epithelial lining were unaffected. Despite overestimation of endocrine cells in thick sections, the estimation of the profile area by image analysis disclosed that the changes in total volume induced by prostaglandins was most likely to be secondary to hyperplasia of endocine cells.
Descriptors		INTESTINAL MUCOSA ENTEROCHROMAFFIN CELLS PROSTAGLANDINS E RATS ANIMALS, LABORATORY HISTOLOGICAL TECHNIQUES DUODENUM SOMATOSTATIN