Author/Editor		Zanconati, F; Cattonar, P; Grandi, G
Title		Histochemical and immunohistochemical methods for demonstration of spirochetes in skin biopsies
Type		članek
Source		Acta Dermatovenerol Alp Pannon Adriat
Vol. and No.		Letnik 3, št. 1-2
Publication year		1994
Volume		str. 99-104
Language		eng
Abstract		Spiral or corkscrew-shaped bacteria are classified as spirochetes. They are not stainable with either Gram or Acid fast techniques, commonly used for bacteria. Histochemical stains for the demonstration of spirochetes in routinely processed skin biopsies with suspected borrelial lesions are based on silver stains. We have tested some filed cases of erythema chronicum migrans and lymphoadenosis benigna cutis employing Warthin-Starry and Steiner and Steiner methods. Spirochetal microorganisms suggesting Lyme Borreliosis have been detected at the dermo-epidermal junction. Immunohistochemical methods are more specific and sensitive and allow the distinction of Spirochetes in their different subtypes, but require monoclonal antibodies not commercially available for routine procedure.
Descriptors		LYME DISEASE SKIN DISEASES BIOPSY BORRELIA BURGDORFERI HISTOCYTOCHEMISTRY ERYTHEMA CHRONICUM MIGRANS LYMPHADENITIS ANTIBODIES, MONOCLONAL