Author/Editor		Stanta, G; Bonin, S; Perin, R
Title		Detection of Borrelia burgdorferi specific DNA in tissues by PCR technology
Type		članek
Source		Acta Dermatovenerol Alp Pannon Adriat
Vol. and No.		Letnik 3, št. 1-2
Publication year		1994
Volume		str. 111-4
Language		eng
Abstract		The amplification of DNA by polymerase chain reaction can be used for detection of Borrelia burgdorferi (Bb) genome in fresh tissues and also in formalin-fixed and paraffin embedded tissues. The method is a multistep procedure. From a histological section 4-8 microns thick enough DNA can be extracted for amplification. The specific sequences of Bb DNA of less than 100 nucleotides are preferred. The amplification is performed in a thermocycler with tube cups heating system. It is concluded that the detection of Bb genome in tissues by PCR is a powerful diagnostic tool, however for economic reasons not suitable as a routine procedure.
Descriptors		BORRELIA BURGDORFERI POLYMERASE CHAIN REACTION LYME DISEASE BIOPSY