Author/Editor		Biškup-Glinšek, Urška; Strle, Franc; Ružić-Sabljić, Eva
Title		Loss of plasmids of Borrelia burgdorferi sensu lato during prolonged in vitro cultivation
Type		članek
Source		Plasmid
Vol. and No.		Letnik 66, št. 1
Publication year		2011
Volume		str. 1-6
Language		eng
Abstract		In the present study we analyzed stability of plasmid content in 34 Borrelia strains of three different species (13 Borrelia afzelii, 10 Borrelia garinii and 11 Borrelia burgodorferi sensu stricto) using pulse field gel electrophoresis (PFGE). During long-term in vitro cultivation consisting of 50 passages, plasmid loss was established in 46% of B. afzelii, 40% of B. garinii and 36% of B. burgdorferi sensu stricto strains. Loss of plasmids occurred as early as between the 5th and 10th passage, affected only plasmids in the range 9-41kb but not plasmids in the range 50-68kb and manifested with the loss of one to up to three plasmids.
Descriptors		BORRELIA BORRELIA BURGDORFERI PLASMIDS ELECTROPHORESIS, GEL, PULSED-FIELD