Author/Editor		Goličnik, Marko
Title		Alternative algebraic rate-integration approach for progress-curve analysis of enzyme kinetics
Type		članek
Source		Eng Life Sci
Vol. and No.		Letnik 12, št. 1
Publication year		2012
Volume		str. 104-8
Language		eng
Abstract		A recent article of Zavrel et al. in this journal [Eng. Life Sci. 2010, 10, 191-200] described a comparison of several computer programs for progress-curve analysis with respect to different computational approaches for parameter estimation. The authors applied both algebraic and dynamic parameter estimations, although they omitted time-course analysis through the integrated rate equation. Recently, it was demonstrated that progress-curve analysis through the integrated rate equation can be considered a simple and useful alternative for enzymes that obey the generalized Michaelis-Menten reaction mechanism. To complete this gap, the time-dependent solution of the generalized Michaelis-Menten equation is here fitted to the progress curves from the Zavrel et al. reference article. This alternative rate integration approach for determining the kinetics parameters of Michaelis-Menten-type enzymes yields the values with the greatest accuracy, as compared with the results obtained by other (algebraic or dynamic) parameter estimations.
Descriptors		ENZYME INDUCTION KINETICS NONLINEAR DYNAMICS ELLAGIC ACID NITROBENZOATES PHENYLACETATES PENICILLIN AMIDASE ESCHERICHIA COLI CATALYSIS HYDROLYSIS