Author/Editor		Cilenšek, Ines; Hercegovac, Amela; Nikolajević-Starčević, Jovana; Vukojević, Katarina; Saraga-Babić, Mirna; Milutinović-Živin, Aleksandra
Title		Polymorphisms of interleukin-4, -10 and 12B genes and diabetic retinopathy
Type		članek
Source		Cent Eur J Biol Print
Vol. and No.		Letnik 6, št. 4
Publication year		2011
Volume		str. 558-64
Language		eng
Abstract		In diabetic retinopathy (DR) and other angiogenesis-associated diseases, increased levels of cytokines, inflammatory cells, and angiogenic factors are present. We investigated the hypothesis that rs2243250 polymorphism of the interleukin 4 (IL-4) gene or rs1800896 polymorphism of the interleukin 10 (IL-10) gene, and rs3212227 polymorphism of the 3' untranslated region (3' UTR) of the interleukin-12 p40 gene (IL12B) may be associated with the development of proliferative diabetic retinopathy (PDR) in Caucasians with type 2 diabetes (DM2). This cross sectional case control study included 189 patients with PDR and 187 patients with type 2 diabetes without PDR. Polymorphisms rs1800896 of the IL-10 gene, rs2243250 of the IL-4 gene, and rs3212227 of IL12B gene were analyzed by ARMS -PCR and RFLP -PCR methods. Multivariate analysis demonstrated the GG genotype of the rs1800896 polymorphism of the IL-10 gene to be associated with increased risk for PDR (OR=1.99; 95% CI=1.11-3.57; P=0.02), whereas the TT genotype of the rs2243250 polymorphism of the IL-4 gene and the AA genotype of the rs3212227 polymorphism of the IL-12 gene were not independent risk factors for PDR. Our findings suggest that the genetic variations at the IL-10 promoter gene might be a genetic risk factor for PDR in Caucasians with type 2 diabetes.
Descriptors		DIABETES MELLITUS, NON-INSULIN-DEPENDENT DIABETIC RETINOPATHY DISEASE PROGRESSION INTERLEUKIN-4 INTERLEUKIN-10 INTERLEUKIN-12 GENOTYPE OPHTHALMOSCOPY POLYMORPHISM, RESTRICTION FRAGMENT LENGTH POLYMERASE CHAIN REACTION MULTIVARIATE ANALYSIS ODDS RATIO