Author/Editor		Žemva, Ž; Kladnik, S; Medvešček, M; Ravnik, M; Bratanič, N; Koselj, M
Title		Hormonska diagnostika sladkorne bolezni
Translated title		Hormonal diagnosis of diabetes mellitus
Type		članek
Source		Farm Vestn
Vol. and No.		Letnik 45, št. 3
Publication year		1994
Volume		str. 231-7
Language		slo
Abstract		In selected groups of patients insulin (total and free), C- peptide, insulin antibodies and insulin receptors were studied with the aim of refining the current diagnosis of diabetes. For hormone determintion radioimmunoassay with 125 I-labeled antigen was used. In a group of 18 patients with type II diabetes, free and total insulin levels were measured before and after therapy in order to determine the effectiveness of the given treatment. After on week of therapy, insulin levels were significantly higher than prior to the institution of therapy while fasting glucose levels were within normal limits. Serum C-peptide was measured at 0 and 6 minutes after the administration of glucogon. Our results agree with those reported in the literature. In type I diabetes, fasting C-peptide was less th. 0.3 nmol/1, but after glucagon stimulation it increased to less th. 0.6 nmol/1. In patients with type II diabetes, C-peptide prior to administration of glucose was greather th. 0.3 nmol/1, and after it greather th. 0.6 nmol/1. Measurements of fasting and postprandial insulin and c-peptide levels in a group of 8 transplant patients with posttransplantation diabetes failed to confirm with certainty insulin resistance in these patients. In patients on insulin treatment, insulin antibody binding was 70 per cent, whereas type II diabetes patients with failure of oral antidiabetic therapy demonstrated notably reduced binding of insulin receptors by 50 per cent as compared to healthy subjects.
Descriptors		DIABETES MELLITUS C-PEPTIDE INSULIN RECEPTORS, INSULIN RADIOIMMUNOASSAY ANTIBODIES DIABETES MELLITUS, NON-INSULIN-DEPENDENT