| Author/Editor | Možina, B; Zupančič, M; Joković, Ž; Prezelj, M; Lukač-Bajalo, J | |
| Title | Glikohemoglobin - validacija in primerjava postopkov | |
| Translated title | Glycohemoglobin assay - validation and comparison | |
| Type | članek | |
| Source | Farm Vestn | |
| Vol. and No. | Letnik 45, št. 3 | |
| Publication year | 1994 | |
| Volume | str. 239-51 | |
| Language | slo | |
| Abstract | Measurements of gycohemoglobin in clinical laboratories afford improved glycemic control and thereby provide for increased metabolic stability in diabetes patients. We studied glycohemoglobin levels (as per cent HbA 1 or total glycated fractions - per cent GHb, depending on the assay methodology) in blood samples from diabetes patients. Glycohemoglobin was assayed by the following techniques: reference ion-exchange high performance liquid chromatografy (HPLC - Bio-Rad, Diamat), affinity chromatography (Abbott, IMx) and turbidimetric inhibition immunoassay (Boehringer Mannheim, Hitachi 717). The two assays atudied were in good agreement with the reference HPLC method (n=105, r=0.9946 for affinity chromatography and n=134, r=0.942 for turbidimetric immunoassay), and showed high reproducibility; the interserial and within-series CVs for both assays were less th. 5 per cent . Both tests are fully automated. For affinity chromatography, whole-blood samples are used, while for turbidimetric immunoassay, blood (capilary or venous= samples are hemolyzed prior to determination. Affinity chromatography shows the lowest susceptibility to interference. On the Abbott IMx analyzer, 24 analyses per hour can be performed. The assay is not specific for HbA 1c, but as it encompasses all glycohemoglobin variants, it provides very accurate information about the metabolic state of diabetes patients. Turbidimetric inhibition immunoassay using monoclonal antibodies is specific for HbA 1c, fraction. Up to 250 assays per hour can be carried out on Hitachi 717 instrument. The advantage of this assay is that it is suited for application on other selective automated analyzer, measuring other parameters of metabolic control in diabetes, such as glucose, fructosamine, lipids, albumin in urine. | |
| Descriptors | HEMOGLOBIN A, GLYCOSYLATED CHROMATOGRAPHY, AFFINITY NEPHELOMETRY AND TURBIDIMETRY CHROMATOGRAPHY, HIGH PRESSURE LIQUID DIABETES MELLITUS |