Author/Editor		Filipovič, Dragana; Pirkmajer, Sergej; Miš, Katarina; Marš, Tomaž; Grubič, Zoran
Title		Expression of glucocorticoid receptors in the regenerating human skeletal muscle
Type		članek
Source		Physiol Res
Vol. and No.		Letnik 60, št. Suppl 1
Publication year		2011
Volume		str. S147-54
Language		eng
Abstract		Many stress conditions are accompanied by skeletal muscle dysfunction and regeneration, which is essentially a recapitulation of the embryonic development. However, regeneration usually occurs under conditions of hypothalamus-pituitary-adrenal gland axis activation and therefore increased glucocorticoid (GC) levels. Glucocorticoid receptor (GR), the main determinant of cellular responsiveness to GCs, exists in two isoforms (GRalpha and GRbeta) in humans. While the role of GRalpha is well characterized, GRbeta remains an elusive player in GC signalling. To elucidate basic characteristics of GC signaling in the regenerating human skeletal muscle we assessed GRalpha and GRbeta expression pattern in cultured human myoblasts and myotubes and their response to 24-hour dexamethasone (DEX) treatment. There was no difference in GRalpha mRNA and protein expression or DEX-mediated GRalpha down-regulation in myoblasts and myotubes. GRbeta mRNA level was very low in myoblasts and remained unaffected by differentiation and/or DEX. GRbeta protein could not be detected. These results indicate that response to GCs is established very early during human skeletal muscle regeneration and that it remains practically unchanged before innervation is established. Very low GRbeta mRNA expression and inability to detect GRbeta protein suggests that GRbeta is not a major player in the early stages of human skeletal muscle regeneration.
Descriptors		RECEPTORS, GLUCOCORTICOID MUSCLE, SKELETAL DEXAMETHASONE RNA, MESSENGER MUSCLE FIBERS STRESS REGENERATION POLYMERASE CHAIN REACTION BLOTTING, WESTERN CELLS, CULTURED