Author/Editor		Keše, Darja; Potočnik, Marko; Matičič, Mojca; Kogoj, Rok
Title		Genotyping of Chlamydia trachomatis directly from urogenital and conjunctiva samples using an ompA gene pyrosequencing-based assay
Type		članek
Source		Fems Immunol Med Microbiol
Vol. and No.		Letnik 63, št. 2
Publication year		2011
Volume		str. 210-6
Language		eng
Abstract		The aim of the present study was to develop and validate a multitarget pyrosequencing-based protocol for basic Chlamydia trachomatis genotyping directly from clinical samples and to characterize the distribution of genotypes among Slovenian sexually active population. The newly developed combination of assays that targets the variable domains VD-I and VD-IV of the C. trachomatisompA gene, was optimized and validated with 11 reference C. trachomatis strains and by comparison to complete ompA conventional sequencing. In addition, 183 clinical specimens which were previously diagnosed as C. trachomatis positive were evaluated by pyrosequencing. The pyrosequencing products showed a 100% match to corresponding sections of the respective conventional ompA sequences. Based on our results the most frequent genotype in urogenital samples was E (51.1%) followed by F (21.4%), G and K (6.9%), D (6.1%), H (3.8%), J (2.3%) and Ia and Ja (0.8%). In conjunctiva samples the genotype distribution was E (63.3%), D and F (13.3%), K (6.7%) and G (3.3%). Pyrosequencing thus proved itself to be a rapid method for C. trachomatis typing, which is important for better understanding the pathogenesis and epidemiology of this pathogen.
Descriptors		UROGENITAL DISEASES CONJUNCTIVAL DISEASES CHLAMYDIA TRACHOMATIS DNA, BACTERIAL GENOTYPE POLYMERASE CHAIN REACTION CHILD