Author/Editor     Lenasi, Tina; Peterlin, Matija B; Dovč, Peter
Title     Distal regulation of alternative splicing by splicing enhancer in equine beta-casein intron 1
Type     članek
Source     RNA
Vol. and No.     Letnik 12, št. 3
Publication year     2006
Volume     str. 498-507
Language     eng
Abstract     The complexity of cotranscriptional splicing is reflected in the coordinated interplay between various cis-elements and transacting factors. In this report, we demonstrated that a cis-element in intron 1 of the equine beta-casein gene (intronic splicing enhancer 1, ISE1) increases the inclusion of all weak exons in its pre-mRNA. The ISE1 also functioned on a hybrid transcript, which was transcribed from the alpha-globin promoter, where it increased the inclusion of the human fibronectin EDA exon and the beta-casein exon 5. The region of ISE1 necessary for its function included the same sequence as is found in some exonic splicing enhancers. Since the ISE1 influenced the splicing of the entire transcript from intron 1, we propose a model for the cotranscriptional splicing of beta-casein mRNA, where the 5' end of the growing transcript remains associated with the C-terminal domain of RNA polymerase II. Thus, the ISE1 remains in close proximity to the mRNA exit groove throughout transcription and influences all weak exons as soon as they are copied.
Descriptors     ALTERNATIVE SPLICING
ANIMALS
BASE SEQUENCE
CASEINS
DNA
EXONS
HORSES
INTRONS
MOLECULAR SEQUENCE DATA
MUTAGENESIS
RNA PRECURSORS