Author/Editor		Zhao, Shu-Hua; Zhao, Fan; Zheng, Jing-Ying; Gao, Li-Fang; Zhao, Xue-Jian; Cui, Man-Hua
Title		Knockdown of stat3 expression by RNAi inhibits in vitro growth of human ovarian cancer
Translated title		Utišanje izražanja stat3 z RNAi zavira rast celic humanega raka jajčnikov in vitro
Type		članek
Source		Radiol Oncol
Vol. and No.		Letnik 45, št. 3
Publication year		2011
Volume		str. 196-203
Language		eng
Abstract		Background. The aim of the study was to investigate the suppressive effects of pSilencer2.1-U6-siRNA-stat3 recombinant plasmids on the growth of ovarian cancer in vitro. Material and methods. Three pairs of DNA template (stat3-1, stat3-2, stat3-3) specific for different target sites on stat3 mRNA were synthesized to reconstruct pSilencer2.1-U6-siRNA-stat3s, which were transfected into SKOV3 cells. The expressions of STAT3, BcL-2, cyclin D1 and C-myc in these cells were detected by Western blot and Northern blot. The cell cycle and the growth were determined by flow cytometry (FCM) and MTT assay, respectively. Cell apoptosis was determined by TUNEL staining. Results. Of the three siRNAs, only siRNA targeting stat3-3 markedly suppressed the protein expression of stat3 in SKOV3 cells; MTT assay and FCM showed that transfection of stat3-3 siRNA could significantly suppress the growth of SKOV3 cells and arrest the cell cycle in vitro. TUNEL staining also showed massive apoptosis in SKOV3 cells transfected with stat3-3 siRNA. Conclusions. pSilencer2.1-U6-siRNA-stat3-3 can significantly inhibit the STAT3 expression in human ovarian cancer cells resulting in the inhibition of the cancer growth and the increase of apoptosis of cancer cells. Key words: RNA interference; stat3; SKOV3 cells; ovarian cancer; apoptosis
Descriptors		OVARIAN NEOPLASMS IMMUNOHISTOCHEMISTRY PLASMIDS RECOMBINANT PROTEINS DNA FRAGMENTATION RNA, MESSENGER TRANSFECTION FLOW CYTOMETRY APOPTOSIS OLIGONUCLEOTIDE PROBES TUMOR CELLS, CULTURED GENE EXPRESSION