Author/Editor		Chen, Hui; Contreras, Xavier; Yamaguchi, Yuki; Handa, Hiroshi; Peterlin, Matija B; Guo, Su
Title		Repression of RNA polymerase II elongation in vivo is critically dependent on the C-terminus of Spt5
Type		članek
Source		PLoS One
Vol. and No.		Letnik 4, št. 9
Publication year		2009
Volume		str. e6918
Language		eng
Abstract		The stalling of RNA polymerase II (RNAPII) at the promoters of many genes, including developmental regulators, stress-responsive genes, and HIVLTR, suggests transcription elongation as a critical regulatory step in addition to initiation. Spt5, the large subunit of the DRB sensitivity-inducing factor (DSIF), represses or activates RNAPII elongation in vitro. How RNAPII elongation is repressed in vivo is not well understood. Here we report that CTR1 and CTR2CT, the two repeat-containing regions constituting the C-terminus of Spt5, play a redundant role in repressing RNAPII elongation in vivo. First, mis-expression of Spt5 lacking CTR1 or CTR2CT is inconsequential, but mis-expression of Spt5 lacking the entire C-terminus (termed NSpt5) dominantly impairs embryogenesis in zebrafish. Second, NSpt5 de-represses the transcription of hsp70-4 in zebrafish embryos and HIVLTR in cultured human cells, which are repressed at the RNAPII elongation step under non-inducible conditions. Third, NSpt5 directly associates with hsp70-4 chromatin in vivo and increases the occupancy of RNAPII, positive transcription elongation factor b (P-TEFb), histone H3 Lys 4 trimethylation (H3K4Me3), and surprisingly, the negative elongation factor A (NELF-A) at the locus, indicating a direct action of NSpt5 on the elongation repressed locus. Together, these results reveal a dominant activity of NSpt5 to de-repress RNAPII elongation, and suggest that the C-terminus of Spt5 is critical for repressing RNAPII elongation in vivo.
Descriptors		GENE EXPRESSION REGULATION, ENZYMOLOGIC ANIMALS CHROMATIN CHROMOSOMAL PROTEINS, NON-HISTONE HEAT-SHOCK PROTEINS HELA CELLS HISTONES MODELS, BIOLOGICAL PROTEIN STRUCTURE, TERTIARY RNA POLYMERASE II TRANSCRIPTION, GENETIC ZEBRAFISH