Author/Editor		Žigon, Polona; Lakota, Katja; Čučnik, Saša; Švec, Tinka; Ambrožič, Aleš; Sodin-Šemrl, Snežna; Kveder, Tanja
Title		Comparison and evaluation of different methodologies and tests for detection of anti-dsDNA antibodies on 889 Slovenian patients' and blood donors' sera
Type		članek
Source		Croat Med J
Vol. and No.		Letnik 52, št. 6
Publication year		2011
Volume		str. 694-702
Language		eng
Abstract		Aim. To evaluate four different commercially available assays for anti-double stranded DNA (dsDNA) detection and compare them with the in-house radioimmunoassay according to Farr (FARR-RIA) in order to select the optimal primary method for use in combination with FARR-RIA. Methods. Sera from 583 consecutive patients sent to our laboratory for routine diagnosis, 156 selected patients with autoimmune diseases (76 systemic lupus erythematosus [SLE] patients and 80 patients with other autoimmune diseases), and 150 blood donors were tested for anti-dsDNA antibodies with two enzyme-linked immunoassays (ELISA), two Crithidia luciliae immunoflourescence tests (CLIFT), and FARR-RIA. The specificities and sensitivities of the tests were calculated and compared. Results. FARR-RIA and CLIFT 2 showed the highest specificity for SLE (100%), with CLIFT 2 showing higher sensitivity (33% vs 47%). Both ELISAs showed higher sensitivities (>53%) than FARR-RIA but lower specificities (<93%), whereas CLIFT 1 showed the lowest overall agreement with FARR-RIA. Conclusion. CLIFT 2 was selected as the primary test for use in combination with FARR-RIA. The use of CLIFT 2 reduced the number of sera that needed to be tested by FARR-RIA, the time needed to report the results, and environmental toxicity, cancerogenicity, and radioactivity.
Descriptors		ANTIBODIES, ANTINUCLEAR AUTOIMMUNE DISEASES RADIOIMMUNOASSAY ENZYME-LINKED IMMUNOSORBENT ASSAY FLUORESCENT ANTIBODY TECHNIQUE ANTIPHOSPHOLIPID SYNDROME LUPUS ERYTHEMATOSUS, SYSTEMIC CROSS-SECTIONAL STUDIES