| Author/Editor | Cilenšek, Ines; Mankoč, Sara; Globočnik-Petrovič, Mojca; Petrovič, Daniel | |
| Title | GSTT1 null genotype is a risk factor for diabetic retinopathy in Caucasians with type 2 diabetes, whereas GSTM1 null genotype might confer protection against retinopathy | |
| Type | članek | |
| Source | Dis Markers | |
| Vol. and No. | Letnik 32, št. 2 | |
| Publication year | 2012 | |
| Volume | str. 93-9 | |
| Language | eng | |
| Abstract | Aim: Substantial data indicate that oxidative stress is involved in the development of diabetic retinopathy (DR). The aim of the present study was to investigate whether the genetic polymorphisms: polymorphic deletions of glutathione S-transferases M1 (GSTM1) and T1 (GSTT1) and Ile105Val of the GSTP1 are associated with DR in Slovenian patients with type 2 diabetes. Methods: In this cross sectional case-control study 604 unrelated Slovene subjects (Caucasians) with type 2 diabetes mellitus were enrolled: 284 patients with DR (cases) and the control group of 320 subjects with type 2 diabetes of more than 10 years' duration who had no clinical signs of DR. Genotypes were determined by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). Results: In our study, the deletion of the GSTM1 was found less frequent in cases with DR than in the controls (27.5% versus 44.4%; P < 0.001), whereas the deletion of GSTT1 was found significantly more often in cases than in the controls (49.3% versus 29.7%; P < 0.001). We did not find statistically significant differences in the genotype distribution in GSTP1 (Ile105Val) polymorphism between cases and controls (40.5% versus 46.0%). Conclusions: We may conclude that individuals homozygous for the deletion of GSTT1 are at an approximately 2-fold-greater risk of DR, whereas the GSTM1 deficiency is associated with lower frequency of DR in type 2 diabetics. | |
| Descriptors | DIABETIC RETINOPATHY RISK FACTORS GENOTYPE DIABETES MELLITUS, NON-INSULIN-DEPENDENT OXIDATIVE STRESS GLUTATHIONE TRANSFERASES POLYMORPHISM (GENETICS) OPHTHALMOSCOPY POLYMERASE CHAIN REACTION CROSS-SECTIONAL STUDIES |