Author/Editor		Goličnik, Marko
Title		Estimation of kinetic parameters for enzyme-Inhibition reaction models using direct time-dependent equations for reactant concentrations
Type		članek
Source		Acta Chim Slov
Vol. and No.		Letnik 59, št. 1
Publication year		2012
Volume		str. 207-11
Language		eng
Abstract		To facilitate the determination of a reaction type and its kinetics constants for reversible inhibitors of Michaelis-Mententype enzymes using progress-curve analysis, I present here an explicit equation for direct curve fitting to full time-course data of inhibited enzyme-catalyzed reactions. This algebraic expression involves certain elementary functions where their values are readily available using any standard nonlinear regression program. Hence this allows easy analysis of experimentally observed kinetics without any data conversion prior to fitting. Its implementation gives correct parameter estimates that are in very good agreement with results obtained using both the numerically integrated Michaelis-Menten rate equation or its exact closed-form solution which is expressed in terms of the Lambert W function.
Descriptors		ENZYME INHIBITORS CATALYSIS KINETICS TIME FACTORS NONLINEAR DYNAMICS MODELS, THEORETICAL