| Author/Editor | Milutinović, Aleksandra; Zorc-Pleskovič, Ruda | |
| Title | Glycogen accumulation in cardiomyocytes and cardiotoxic eff ects after 3NPA treatment | |
| Type | članek | |
| Source | Bosnian journal of basic medical sciences | |
| Vol. and No. | Letnik 12, št. 1 | |
| Publication year | 2012 | |
| Volume | str. 15-9 | |
| Language | eng | |
| Abstract | Mitochondrial toxin 3-nitropropionic acid (3NPA) is a neurotoxin that inhibits the activity of succinate dehydrogenase, a key enzyme of oxidative energy production, and characteristically provokes neurodegeneration in the striatum, resembling Huntington's disease. 3NPA also aff ects the activity of glycogen-sinthase-kinase-3b (GSK-3b), an enzyme implicated in glycogen synthesis and in signal transduction. The aim of this study was to evaluate cardiac glycogen content and histopathological changes in the hearts of rats after subchronic treatment with 3NPA. Female adult Wistar rats were treated daily with 30mg/kg of 3NPA subcutaneously 8 days. The control group was treated with normal saline for 8 days. For the comparison of measured parameters between groups we used the Student's t-test (p<0,05). The stereological evaluation of glycogen content in histological sections of the heart was processed with periodic acid-Schiff (PAS). Histochemical procedure showed a signifi cant accumulation of glycogen granules in the 3NPA group (0,28mm3/mm3+-0,022), whereas the hearts of control animals were nearly devoid of glycogen granules (0,002mm3/mm3+- 0,001). Haematoxylin-eosin histological staining showed diff use swelling of cardiomyocytes (3NPA=15,98microm +- 1,649; saline=13,45gammamicro +- 0,786m), loss of cell cross-striations, lower myofi bril volume fraction (3NPA=0,3922mm3/mm3 +- 0,0230, saline=0,4550mm3/mm3 +- 0,0083), and mononuclear infi ltration in the interstitial tissue, mostly along the blood vessels. Sirius red staining showed fi brosis of the heart (3NPA=0,0531mm3/mm3 +- 0,0090, saline=0,0135mm3/mm3 +- 0,0051). TUNEL staining showed TUNEL-positive cells in the 3NPA group (2,04cells/mm2 +- 0,92) and almost no TUNEL-positive cells in the saline group (0,27cells/mm2 +- 0,14). (Abstract truncated at 2000 characters) | |
| Descriptors | HEART PROPIONIC ACIDS MYOFIBRILS HISTOCYTOCHEMISTRY APOPTOSIS HIPPOCAMPUS RATS, WISTAR GLYCOGEN |