Author/Editor		Knap, Nataša; Korva, Miša; Dolinšek, Vesna; Sekirnik, Mojca; Trilar, Tomi; Avšič-Županc, Tatjana
Title		Patterns of tick-borne encephalitis virus infection in rodents in Slovenia
Type		članek
Source		Vector Borne Zoonotic Dis
Vol. and No.		Letnik 12, št. 3
Publication year		2012
Volume		str. 236-42
Language		eng
Abstract		Tick-borne encephalitis virus (TBEV) is the most important causative agent of arboviral infection in Europe, causing neurologic symptoms. The incidence of the disease has greatly increased over the past decades, and in the meantime, some changes in spatial distribution of TBE cases have been observed. Therefore, it is important to recognize the distribution of endemic areas, to use preventive measures successfully. In this study, rodents from all over Slovenia were evaluated as suitable sentinels for TBEV distribution. Rodents from four species (Myodes glareolus, Apodemus flavicollis, Apodemus sylvaticus, and Apodemus agrarius) were screened for the presence of TBEV antibodies with immunofluorescence assay; the antibodies were detected in 5.9% of sera. The prevalence of infection varied according to the rodent species and according to the region of trapping. Select rodents were also screened for the presence of TBEV RNA in several organs. Both analyses showed higher rate of infection in bank voles, which also produced higher titers of anti-TBEV antibodies and a higher TBEV RNA viral load compared with mice. The regional prevalence of infection in rodents can be correlated with the incidence of disease. Molecular results indicate that the virus can be detected in the organs of the rodents for longer periods, indicating prolonged infections of the rodent hosts by the virus. Rodents can therefore be used as a useful indicator of the circulation of TBEV in an area.
Descriptors		MURIDAE ENCEPHALITIS VIRUSES, TICK-BORNE ENCEPHALITIS, TICK-BORNE POLYMERASE CHAIN REACTION RNA, VIRAL VIRAL LOAD SLOVENIA