Author/Editor		Parviainen, Markku; Kelo, Eira; Sirola, Helena; Vaheri, Antti; Vapalahti, Olli; Avšič-Županc, Tatjana; Mononen, Ilkka
Title		Detection of acute hantavirus infections using novel instrument-readable rapid tests
Type		članek
Source		European infectious disease
Vol. and No.		Letnik 5, št. 1
Publication year		2011
Volume		str. 35-7
Language		eng
Abstract		Hantaviruses infecting humans in Eurasia include the Hantaan, Seoul, Puumala viruses and the closely related Dobrava and Saaremaa viruses. These viruses are causative agents of haemorrhagic fever with renal syndrome (HFRS), which is recognised as a severe healthcare problem in several countries. Routine diagnosis of hantavirus infection relies on serology. We describe a novel instrument-readable, antigen-based, rapid test for detection of immunoglobulin M (IgM) antibodies present in the serum of patients with acute Puumala and Dobrava or Hantaan virus infections. The assays have been validated and evaluated using serum samples confirmed as hantavirus IgM negative, and sera of patients with acute infections of the various hantaviruses. Infections caused by all pathogenic hantaviruses are detected with high sensitivity (96.3-97.1%) and specificity (95.2-98.4%).
Descriptors		HANTAVIRUS HEMORRHAGIC FEVER WITH RENAL SYNDROME ANTIBODIES, VIRAL IGM DIAGNOSTIC TESTS, ROUTINE SENSITIVITY AND SPECIFICITY