Author/Editor		Knez, Jure; Vlaisavljević, Veljko; Kovačič, Borut
Title		Intracytoplasmatic sperm injection (ICSI) in infertility treatment: analysis of 3.339 consecutive cycles
Type		članek
Source		Gynaecol Perinatol
Vol. and No.		Letnik 20, št. 2
Publication year		2011
Volume		str. 65-71
Language		eng
Abstract		Objective. Introduction of intracytoplasmic sperm injection (ICSI) represented an important step in male infertility treatment. Today it is used in more than half of assisted reproductive procedures. Our aim was to investigate the paternal influence on ICSI procedure outcome. Methods. A total of 3.339 ICSI cycles were included in the retrospective analysis. After exclusion of female factor infertility related cycles, 919 cases remained for detailed analysis. Patients were classified according to sperm analysis and ICSI outcome was evaluated. Results. In 3.339 cycles, oocyte fertilization rate was 74 %. There were 1.134 clinical pregnancies (34 % per cycle) and 942 deliveries (28 % per cycle). Comparing ICSI with testicular spermatozoa to ICSI with ejaculated spermatozoa, lower fertilization rate (64 % vs. 73 %, p<0.05), blastulation rate (40 % vs. 53 %, p<0.05) and day 5 blastocyst transfer rate (65 % vs. 78 %, p<0.05) can be observed. Among the cycles using ejaculated spermatozoa, cryptozoospermia shows a negative effect on fertilization rate (50 %, p<0.05) and blastocyst development (43 %, p<0.05), but does not influence clinical pregnancy (44 %, p<0.05) and delivery rates (41 %, p<0.05). Conclusion. Very low sperm concentration and motility can predict low fertilization rate and slow embryo development after ICSI. The possibility of high quality embryo selection for transfer on day 5 allows high delivery rates in different groups of patients treated with ICSI using ejaculated or testicular spermatozoa.
Descriptors		INFERTILITY, MALE SPERM MOTILITY FERTILIZATION IN VITRO TREATMENT OUTCOME