Author/Editor		Zupančič, Tina; Ozir, Mateja; Toermae, Hans; Komel, Radovan; Liović, Mirjana
Title		Keratinocyte-based cell assays: their potential pitfalls
Type		članek
Source		Arch Dermatol Res
Vol. and No.		Letnik 304, št. 9
Publication year		2012
Volume		str. 765-8
Language		eng
Abstract		As an in vitro model system, patient-derived epidermolysis bullosa simplex keratinocytes have had an immense impact on what we know today about keratin filament function and their role in disease development. In the absence of gene therapy, screening com pound libraries for new or better drug s is another approach to improve existing treatments for genodennatoses. However in this study, we report of the potential pitfalls when using this type of cell lines as a reporter system. When cell lines with different genetic backgrounds are being used in cellbased assays, the greatest obstacle is to determine the most appropriate culture conditions (i.e., the composition of medium, number of cells plated and number of day s in culture). We demonstrate how culture conditions can greatly interfere with the cellular response in cell-based assays (cell proliferation, metabolic activity and migration), potentially al so giving rise to misleading data.
Descriptors		EPIDERMOLYSIS BULLOSA SIMPLEX KERATINOCYTES CELL DIVISION CELL MOVEMENT CELLS, CULTURED