Author/Editor		Howl, John; Matou-Nasri, Sabine; West, David C; Zorko, Matjaž
Title		Bioportide: an emergent concept of bioactive cell-penetrating peptides
Type		članek
Source		Cell Mol Life Sci
Vol. and No.		Letnik 69, št. 17
Publication year		2012
Volume		str. 2951-66
Language		eng
Abstract		Cell-penetrating peptides (CPPs) have proven utility for the highly efficient intracellular delivery of bioactive cargoes that include peptides, proteins, and oligonucleotides. The many strategies developed to utilize CPPs solely as pharmacokinetic modifiers necessarily requires them to be relatively inert. Moreover, it is feasible to combine one or multiple CPPs with bioactive cargoes either by direct chemical conjugation or, more rarely, as non-covalent complexes. In terms of the message-address hypothesis, this combination of cargo (message) linked to a CPP (address) as a tandem construct conforms to the sychnological organization. More recently, we have introduced the term bioportide to describe monomeric CPPs that are intrinsically bioactive. Herein, we describe the design and biochemical properties of two rhegnylogically organized monometic CPPs that collectively modulate a variety of biological and pathophysiological phenomena. Thus, camptide, a cell-penetrant sequence located within the first intracellular loop of a human calcitonin receptor, regulates cAMP-dependent processes to modulate insulin secretion and viral infectivity. Nosangiotide, a bioportide derived from endothelial nitric oxide synthase, potently inhibits many aspects of the endothelial cell morphology and movement and displays potent anti-angiogenic activity in vivo. We conclude that, due to their capacity to translocate and target intracellular signaling events, bioportides represent an innovative generic class of bioactive agents.
Descriptors		ASTROCYTOMA CELL MEMBRANE PERMEABILITY PEPTIDES RECEPTORS, CALCITONIN INSULIN RATS NITRIC-OXIDE SYNTHASE SIGNAL TRANSDUCTION BIOLOGICAL TRANSPORT, ACTIVE ENDOTHELIUM, VASCULAR CELL SURVIVAL CELL DIVISION HEPATITIS C CYCLIC AMP GUANOSINE TRIPHOSPHATE TRANSLOCATION (GENETICS) CELLS, CULTURED