Author/Editor		Alibegović, Armin; Balažic, Jože; Petrovič, Danijel; Kregar-Velikonja, Nevenka; Blagus, Rok; Šuput, Dušan; Drobnič, Matej
Title		The optimal combination of cartilage source and apparatus for long-term In vitro chondrocyte viability analysis
Type		članek
Source		J Forensic Sci
Vol. and No.		Letnik 57, št. 6
Publication year		2012
Volume		str. 1601-7
Language		eng
Abstract		Most studies of long-term chondrocytes survival were for tissue banks. They showed a gradual reduction in the viable chondrocytes percentage as a function of time and ambient temperature, but the samples were harvested under optimal conditions. The aim of our study was to determine the most reliable combination of cartilage source and assay for the in vitro postmortem chondrocyte viability analysis in the conditions that imitate a dead body. Osteochondral cylinders were procured from femoral condyles and talar trochleas of three male donors and stored in the cell culture media at 4 +/- 2 degrees C and 23 +/- 2 degrees C. The samples were analyzed by a cell viability analyzer and a confocal laser scanning microscope (CLSM) initially 24-36 h after death and then in 4-week intervals. The results reconfirmed the significant influence of time (p = 0.0002), but not of the temperature (p = 0.237). The largest reproducibility was presented for the knee joint and the CLSM.
Descriptors		FORENSIC MEDICINE CARTILAGE, ARTICULAR POSTMORTEM CHANGES CELL SURVIVAL TEMPERATURE TIME FACTORS MICROSCOPY, CONFOCAL