Author/Editor		Kariž, Stojan; Nikolajević-Starčević, Jovana; Petrovič, Danijel
Title		Association of manganese superoxide dismutase and glutathione S-transferases genotypes with myocardial infarction in patients with type 2 diabetes mellitus
Type		članek
Source		Diabetes Res Clin Pract
Vol. and No.		Letnik 98, št. 1
Publication year		2012
Volume		str. 144-50
Language		eng
Abstract		Aim: In the present study we investigated the association between genetic polymorphisms with functional effects on redox regulation: Val16Ala of manganese superoxide dismutase (MnSOD), polymorphic deletions of glutathione S-transferases M1 (GSTM1) and T1 (GSTT1) and Ile105Val of glutathione S-transferase P1 (GSTP1) and myocardial infarction (MI) in a group of patients with type 2 diabetes mellitus. Methods: The study population consisted of 463 Caucasian subjects with type 2 diabetes mellitus of more than 10 years' duration: 206 patients with MI and 257 patients with no history of coronary artery disease (CAD). Genotypes were determined by polymerase chain reaction (PCR) with restriction fragment length polymorphism (RFLP) and with multiplex PCR. Results: The genotype distributions of tested single nucleotide polymorphisms did not show significant difference between cases and controls. After adjustment for age, gender, smoking, BMI, duration of diabetes and lipid parameters carriers of GSTM1/GSTT1-null haplotype showed an increased risk for MI (OR=3.22, 95% CI 1.37-5.04, p=0.03). Conclusion: The GSTM1/GSTT1 haplotype might be a genetic risk factor for MI in patients with type 2 diabetes mellitus.
Descriptors		MYOCARDIAL INFARCTION DIABETES MELLITUS, NON-INSULIN-DEPENDENT OXIDATIVE STRESS SUPEROXIDE DISMUTASE MANGANESE GLUTATHIONE TRANSFERASES GENOTYPE POLYMORPHISM, RESTRICTION FRAGMENT LENGTH POLYMERASE CHAIN REACTION HAPLOTYPES