Author/Editor		Makuc, Jana; Petrovič, Danijel
Title		Diabetic nephropathy in type 2 diabetes: MPO T-764C genotype is associated with oxidative stress
Type		članek
Source		Cent Eur J Biol Print
Vol. and No.		Letnik 7, št. 6
Publication year		2012
Volume		str. 964-72
Language		eng
Abstract		Background: Oxidative stress is a single mechanism relating all major pathways responsible for diabetic damage and plays an important role in diabetes development, progression and related vascular complications. To investigate the impact of oxidative stress related gene polymorphisms on development of diabetic nephropathy (DN), we tested 7 polymorphic variants that could hypothetically affect the ability of the antioxidant defense system and thus accelerate oxidative stress. Methodology: 197 Slovenian (Caucasian) type 2 diabetic (T2D) patients, age 34-83, classified into two groups according to the presence of DN, were tested for SOD2 Val16Ala (rs4880), p22 phox C242T (rs4673), CAT C-262T (rs1001179), MPO T-764C (rs2243828), GSTP1 Ile105Val (rs1695), GSTT1 and GSTM1 deletion polymorphisms using PCR, RFLP and qPCR. Oxidative stress was assessed through serum 8-hydroxy-2-deoxyguanosine (8-OHdG) level. Results were analyzed using ANOVA, Chi-square test and multivariate logistic regression. Results and Conclusions: Despite the commonly recognized link between oxidative stress and diabetes and its complications we found no association between the selected polymorphisms and DN. However, we confirmed an association between oxidative stress level and MPO T-764C genotype, which was tested in relation to DN for the first time.
Descriptors		DIABETES MELLITUS, NON-INSULIN-DEPENDENT DIABETIC NEPHROPATHIES GENOTYPE OXIDATIVE STRESS POLYMORPHISM (GENETICS) DEOXYGUANOSINE ANALYSIS OF VARIANCE MULTIVARIATE ANALYSIS