Author/Editor		Čelesnik, N; Vesel, T; Rijavec, M; Šilar, M; Eržen, R; Košnik, M; Kloft-Žitnik, SE; Avčin, T; Korošec, P
Title		Short-term venom immunotherapy induces desensitization of FcepsilonRI-mediated basophil response
Type		članek
Source		Allergy
Vol. and No.		Letnik 67, št. 12
Publication year		2012
Volume		str. 1594-600
Language		eng
Abstract		Background: The precise immunological mechanisms for the early clinical protection of venom immunotherapy (VIT) have not yet been explained. Our aim was to evaluate whether high-affinity IgE receptor (FcepsilonRI) and the related basophil function have a role in the induction of short-term VIT protection. Methods: We included 60 adults and 48 children. Basophil threshold sensitivity (CD-sens) to anti-FcepsilonRI stimulation, and FcepsilonRI gene and cell-surface expression were assessed at the beginning and just before the first maintenance dose (MD) of 100 mug of ultra-rush VIT (day 5) and at the beginning, during buildup, and just before the first MD of 70 mug and of 100 mug of semi-rush VIT (weeks 1-2 and 5). Results: We demonstrated a significant reduction in CD-sens to anti-FcepsilonRI stimulation before the first MD in both ultra-rush and semi-rush VIT in all included subjects. FcepsilonRI gene and/or cell-surface expression was decreased in 34-100% of subjects, with different dynamics between VIT protocols. Conclusion: We found a marked desensitization of FcepsilonRI-activated basophils after short-term VIT. This suppression, which could be highly relevant for the development of early protective mechanisms, might be also related to the changes at the level of FcepsilonRI expression.
Descriptors		INSECT BITES AND STINGS BEE VENOMS WASP VENOMS HYPERSENSITIVITY DESENSITIZATION, IMMUNOLOGIC HYMENOPTERA BASOPHIL DEGRANULATION TEST GENE EXPRESSION RECEPTORS, IGG