Author/Editor		Filipič, B; Rozman, S; Carlsson, K; Cencič, A
Title		Porcine interferon gamma (PoIFN Gamma)
Translated title		Prašičji interferon gama (PoIFN Gamma)
Type		članek
Source		Zdrav Vestn
Vol. and No.		Letnik 63, št. Suppl 2
Publication year		1994
Volume		str. II-31-II-3
Language		eng
Abstract		Background. The experiments were performed to study the induction, partial purification and antigenic analysis of PoIFN Gamma, as well as to prepare the potentially clinically useful form for in vivo experiments. Methods. Porcine buffy-coat was resuspended in the Eagle's medium in concentration of 10 on power 7 - 10 on power 8 cells/ml. The induction was performed by the LCL (Lens culinaris lectin) in concentration of 25 micro g/ml. PoIFN Gamma was partially, purified by the adsorption/ elution on the water glass, and than further on with the column chromatography on the CPG (control pore glass). The IFN obtained was characterized through the following tests: antiviral/antiproliferative activity, stability at pH 2.0 and 56 degrees C, protein content, serological tests and Agarose gel electrophoresis. Results. Using the LCL as inducer, ahout 36 000 units of the antiproliferative activity/ml of PoIFN Gamma were obtained. Through the adsorbtion/elution technique on the waterglass, the purification degree between 7 and 10 times was found. This was enlarged 100 times after an chromatography/rechromatography on the CPG (control pore glass). The agarose gel electrophoretic profile shows that the most of the interferon activity can be found in the Alpha-2 like fraction. The results of the serological analysis shows that the cross reactivity between the PoIFN Gamma and HuIFN Alpha but not with HuIFN Gamma can be found. Conclusions. The studied PoIFNGamma shows the cross reactivity with HuIFN Alpha as well as with its natural subtype Alpha 2. Further experiments with purified PoIFN Gamma together with G terminal sequence comparison are expected to show the real level of similarity between the Porcine and Human IFNs.
Descriptors		INTERFERON TYPE II INTERFERON INDUCERS INTERFERON-ALPHA CHROMATOGRAPHY ELECTROPHORESIS, AGAR GEL