Author/Editor		Stancic, Alma; Sarajlić, Nermin; Balažic, Jože; Zupanc, Tomaž
Title		Vitreous humor between-eyes difference
Type		članek
Vol. and No.		Letnik 7, št. 7
Publication year		2013
Volume		str. 2067-2072
ISSN		1840-2291 - HealthMED : Journal of Society for development in new net environment in B&H
Language		eng
Abstract		Objective: The aim of this study is to analyze post-mortem concentration of potassium, sodium and chloride in vitreous of same pair of the eyes, aspirated and analyzed at the same time. Material and Methods: This is retrospective study of biochemical analyses of vitreous samples of n=100 cases, collected at autopsies which were performed at Institute of forensic medicine, Medical faculty, University of Ljubljana. All samples from right and left eye are collected and analyzed at the same time. In all cases included in this study potassium, sodium and chloride concentrations were measured. All vitreous samples are collected respecting Coes method. Approximately 2-3 ml of vitreous was aspirated in each case. Analyses were performed using Olympus AU 400 automatic analyzer, by Ion-selective methodology. Results: The results of the present study suggest that concentration differences between eyes, at identical post-mortem interval, are not significant. The highest one sample test result is measured for potassium, which means potassium left right concentration differences are close to zero, which is default value of the test. For chloride the result of one sample test is the smallest, however, p value of one sample test is higher than 0, 05 which means there are no statistically significant difference in their between eyes concentrations. All coefficients of correlation are high and significant, which confirms there are no statistically significant between eyes difference for concentration of potassium, sodium and chloride. Conclusion: Present study resolves the issue of between eyes difference at identical post-mortem interval for vitreous electrolytes potassium, sodium and chloride. The results of this study clearly suggest these differences to be insignificant and therefore the validity of post-mortem vitreous humor analysis in forensic pathology applications cannot be questioned solely on the basis of these differences.
Keywords		steklovina elektroliti biokemična analiza vitreous electrolytes biochemical analyses