Author/Editor		Syafrizayanti, Christian; Hoheisel, Jörg; Kastelic, Damjana
Title		Methods for analyzing and quantifying protein-protein interaction
Type		članek
Vol. and No.		Letnik 11, št. 1
Publication year		2014
Volume		str. 107-120
ISSN		1744-8387 - Expert review of proteomics
Language		eng
Abstract		Genome sequencing has led to the identification of many proteins, which had not been recognized before. In consequence, the basic set of human proteins is generally known. Far less information, however, exists about protein-protein interactions, which are required and responsible for cellular activities and their control. Many protein isoforms that result from mutations, splice-variations and post-translational modifications also come into play. Until recently, interactions of only few protein partners could be analyzed in a single experiment. However, this does not meet the challenge of investigating the highly complex interaction patterns in cellular systems. It is made even more demanding by the need to determine the intensity of interactions quantitatively in order to properly understand protein interplay. Currently available techniques vary with respect to accuracy, reliability, reproducibility and throughput and their performances range from a mere qualitative demonstration of binding to a quantitative characterization of affinities. In this article, an overview is given of the methodologies available for analysis of protein-protein interactions.
Keywords		proteomics methods for analyzing interactions proteomika metode za analizo interakcije