Author/Editor		Alibegović, Armin; Balažic, Jože; Petrovič, Danijel; Hribar, Gorazd; Blagus, Rok; Drobnič, Matej
Title		Viability of human articular chondrocytes harvested postmortem
Type		članek
Publication year		2014
ISSN		0022-1198 - Journal of forensic sciences
Language		eng
Abstract		Different studies of long-term chondrocytes viability have shown a gradual reduction as a function of time and ambient temperature. The aim of our in vitro study was to establish chondrocyte postmortem viability curves for 4⁰C, 11⁰C, 23⁰C, 35⁰C during 63 days after the donors' death. Osteochondral cylinders were procured from the knees of 16 male donors (2047 years), stored in preservation media that was not changed, and analyzed in 3-day intervals using a confocal laser scanning microscope. A significant influence of time on viability was found from Day 9 (p = 0.0029) and onwards (p < 0.0001). The lowest overall chondrocyte viability was at 35⁰C, followed by 4⁰C (p < 0.0001). The conditions used in this in vitro analysis suggest that similar viabilities may occur while in situ in the decedent. Further studies of chondrocyte viability from individuals with known postmortem intervals may show premise to help evaluate time since death in the late postmortem interval.
Keywords		forensic science forensic pathology confocal laser scanning forenzična znanost forenzična patologija konfokalno lasersko skeniranje