Author/Editor		Zemljič Jokhadar, Špela; Majhenc, Janja; Svetina, Saša; Batista, Urška
Title		Positioning of integrin [beta]1, caveolin-1 and focal adhesion kinase on the adhered membrane of spreading cells
Type		članek
Vol. and No.		Letnik 37, št. 12
Publication year		2013
Volume		str. 1276-1284
ISSN		1520-5207 - The journal of physical chemistry. B
Language		eng
Abstract		We have investigated the relationship between the spreading of anchorage-dependent cells and the surface-density distribution of plasma membrane adhesion proteins. The surface positioning and density of integrin [beta]1, caveolin-1 (cav-1), the phosphorylated caveolin-1 (p-cav-1) and the focal adhesion kinase (FAK) located on the adhering cell membrane (ACM) of HUVEC cells was studied. Imaging with TIRF microscopy was used, which enabled us to observe a few-nanometers-thin section of the cell above the plasma membrane in combination with image-based analyses. Integrin [beta]1 and cav-1 have spatial interdependence on the ACM. Cells treated with substances that act on cell spreading caused changes in the size of the ACM area, as well as a redistribution of several proteins under investigation. Changes to the ACM area correlated positively with those to the surface density of the cav-1. The high integrin [beta]1 and the low cav-1 surface density, and vice versa, following the treatments show that the presence of one of them not only spatially excludes, but also reduces, the occurrence of the other protein on the ACM, which indicates a regulative mechanism between integrin [beta]1 and cav-1.
Keywords		caveolin-1 cytoskeleton surface density caveolin-1 citoskelet površinska gostota