Author/Editor		Veinović, Gorana; Cerar, Tjaša; Strle, Franc; Ružić-Sabljić, Eva
Title		Influence of MKP medium stored for prolonged periods on growth and morphology of Borrelia afzelii, Borrelia garinii, and Borrelia burgdorferi sensu stricto
Type		članek
Vol. and No.		Letnik 122, št. 3
Publication year		2014
Volume		str. 230-235
ISSN		0903-4641 - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
Language		eng
Abstract		Modified Kelly-Pettenkofer (MKP) medium is one of the several media used for isolation and cultivation of Borrelia. The aim of the study was to assess whether particular Borrelia species (B. afzelii, B. garinii, and B. burgdorferi sensu stricto) have the ability to grow in MKP medium stored at +4 ⁰C for periods for 1 month up to 1 year, and how prolonged storage may influences Borrelia growth and morphology. The growth of Borrelia was evaluated after 5 days of incubation at 33 ⁰C: cell count per mL, morphology, and motility were assessed. The results of this study showed that the duration of storage of MKP medium had statistically significant influence on growth of B. afzelii (p = 0.021) and B. garinii (p = 0.004), but not on growth of B. burgdorferi sensu stricto (p = 0.204), whereas duration of storage of the medium had no impact on Borrelia morphology and motility. The results of the study indicate that medium stored for more than 1 and up to 12 months supports Borrelia growth.
Keywords		borelia cultivation MKP medium storage