| Author/Editor | Dernovšek, MZ | |
| Title | Vpliv nekaterih nevroaktivnih snovi na podgane s spontanimi epileptičnimi napadi | |
| Type | monografija | |
| Place | Ljubljana | |
| Publisher | Medicinska fakulteta | |
| Publication year | 1995 | |
| Volume | str. 48 | |
| Language | slo | |
| Abstract | Several chemical agents were used to produce animal models of seizures. Kainic acid-induced seizures are claimed to be a good animal model for complex partial epilepsy in humans. Kainic acid (KA), a glutamate analog with potent neuroexcitant and neurotoxic properties, injected systemically (10 mg/kg or higher doses) causes acute limbic-onset partial seizures that secondarily generalize and progress to epileptic status. KA-induced epileptic status provokes neuronal death and necrosis in different parts of the limbic system: the hippocompus, the amygdala, the piriform cortex. In four to six weeks after epileptic status spontaneous recurrent seizures (SRS) develop in up to 60 percent of animals. To evaluate the effect of interruption of KA-induced epileptic status with diazepam the survival rate, the number of SRS rats and the activity of choline acetyltransferase (ChAT) in amygdala-periform cortex samples were estimated. The SRS rats were tested for their susceptibility to KA applying the equal to the priming dose, used previously in the same animals for the induction of epileptic status. The SRS rats were pretreated with diazepam, MK-801 (non-competitive NMDA glutamate receptor antagonist) or scopolamine before KA and the modification of KA-induced behaviour was recorded and analysed. The treatment with diazepam 90 min after the onset of KA-induced epileptic status stopped the seizure activity in less than 10 minutes, but did not prevent the destruction of cholinergic structures in the amygdala-piriform cortex samples (the activity of ChAT was lowered). The interruption of KA-induced epileptic status with diazepam enhanced the survival of the rats and increased the number of surviving SRS rats. KA (10 mg/kg s.c.) administered to SRS rats did not induce epileptic status.(trunc.) | |
| Descriptors | EPILEPSY KAINIC ACID DIAZEPAM AMYGDALOID BODY CHOLINE ACETYLTRANSFERASE RATS ANIMALS, LABORATORY EPILEPSY, PARTIAL EPILEPSY, TEMPORAL LOBE DIZOCILPINE MALEATE SCOPOLAMINE DISEASE MODELS, ANIMAL |