Author/Editor		Allmeier, H; Črešnar, B; Greck, M; Schmitt, R
Title		Complete nucleotide sequence of Tn1721: gene organization and a novel gene product with features of a chemotaxis protein
Type		članek
Source		Gene
Vol. and No.		Letnik 111, št. 1
Publication year		1992
Volume		str. 11-20
Language		eng
Abstract		The complete 11,139-nucleotide sequence of transposon Tn1721 has been determined. It contains three 38-bp inverted repeats, and (in this order) a new orfI, a resolution site (res), genes encoding resolvase (tnpR), transposase (tnpA), tetracycline-resistance (TcR) repressor (tetR), TcR (tetA) and a truncated transposase gene (tnpA'). The modulator origin of Tn1721 from at least three separate sources is supported by the distinctive codon usages of orfI, tnpR/tnpA and tetR/tetA, and by sequence similarities with Tn501 (tnpR/tnpA) and RP1 (tetR/tetA). The ORFI-encoded 56-kDa polypeptide exhibits features of a methyl-accepting chemotaxis protein (MCP) with a conserved signal domain and a potential transmembrane domain; this polypeptide cross-reacts with anti-MCP antiserum. Like chemotaxis genes, orfI is transcribed from a sigma 28-like promoter. The overexpressed orfI gene product interferes with MCP-dependent chemotaxis suggesting that it completes for soluble transducer protein(s) in the cell. The potential selective advantage of this novel transposon-borne gene is discussed.
Descriptors		BACTERIAL PROTEINS CHEMOTACTIC FACTORS DNA INSERTION ELEMENTS MEMBRANE PROTEINS AMINO ACID SEQUENCE BASE SEQUENCE BLOTTING, WESTERN DNA EVOLUTION MOLECULAR SEQUENCE DATA OPEN READING FRAMES PROMOTER REGIONS (GENETICS) RESTRICTION MAPPING SEQUENCE ALIGNMENT SIGNAL TRANSDUCTION TRANSCRIPTION, GENETIC