Author/Editor		Črešnar, B; Mages, W; Muller, K; Salbaum, JM; Schmitt, R
Title		Structure and expression of a single actin gene in Volvox carteri
Type		članek
Source		Curr Genet
Vol. and No.		Letnik 18, št. 4
Publication year		1990
Volume		str. 337-46
Language		eng
Abstract		Southern blot analysis of Volvox carteri DNA indicated the presence of a single actin gene; the nucleotide sequence of that gene is reported here. In comparison with plant animal and fungal actins, the derived primary structure of 377 amino acids is highly conserved yielding similarity values of 79 percent to 94 percent (including non-identical conservative exchanges). In contrast, the intron structure of the gene is highly unusual: in addition to one intron in the 5' untranslated region (ten nucleotides upstream of the initiator ATG), it has eight introns in the coding region, only three of which are in locations where introns have previously been reported. Transcription starts 26 nucleotides downstream of the putative TATA box and 70 nucleotides downstream of a conspicuous CCAAT motif. A potential polyadenylation signal, TGTAA, is located 366 nucleotides downstream of the terminator TAA. Northern hybridization indicates that the actin gene is transcribed throughout the Volvox life cycle with only a slight depression during the release of juveniles from mother spheroids. This pattern of gene expression suggests that actin may assume various functional roles in the differentiation and growth of Volvox.
Descriptors		ACTINS ALGAE, GREEN GENES, STRUCTURAL, PLANT AMINO ACID SEQUENCE BASE SEQUENCE CLONING, MOLECULAR EXONS INTRONS MOLECULAR SEQUENCE DATA NUCLEOTIDE MAPPING TRANSCRIPTION, GENETIC