Author/Editor     Jezernik, K; Pipan, N
Title     Cytochemical localization of carbohydrates in intercalated duct and acinar cells of mouse parotid gland
Type     članek
Source     Histochem J
Vol. and No.     Letnik 21, št. 3
Publication year     1989
Volume     str. 131-5
Language     eng
Abstract     The glycoconjugate composition of mouse intercalated duct and acinar cells of parotid gland has been compared. Mucins containing 1,2-glycols were demonstrated by the tannic acid-uranyl acetate technique. Hexose residues of glycoconjugates were identified using ferritin conjugated with Canavalia ensiformis agglutinin (Con A), Triticum vulgare or wheat germ agglutinin (WGA), Ricinus communis I agglutinin (RCA-I), Phaseolus vulgaris agglutinin (PHA-E) and Arachis hypogaea agglutinin (PNA). Whereas qualitative and quantitative differences were observed in sugar residues of secretory granules in intercalated duct and acinar cells, apical plasmalemmae were labelled sparsely and similarly. This indicates that the glycocalyx composition of apical plasmalemmae in the parotid acinar and intercalated duct cells is little influenced by secretory granule composition.
Descriptors     CARBOHYDRATES
PAROTID GLAND
CELL MEMBRANE
CYTOPLASMIC GRANULES
HISTOCYTOCHEMISTRY
MICE
MICROVILLI
PAROTID GLAND
STAINS AND STAINING