Author/Editor		Kokalj-Vokač, N; Almeida, A; Viegas-Pequignot, E; Jeanpierre, M; Malfoy, B; Dutrillaux, B
Title		Specific induction of uncoiling and recombination by azacytidine in classical satellite-containing constitutive heterochromatin
Type		članek
Source		Cytogenet Cell Genet
Vol. and No.		Letnik 63, št. 1
Publication year		1993
Volume		str. 11-5
Language		eng
Abstract		Azacytidine (ACR) is known to induce uncoiling and somatic association involving the constitutive heterochromatin of human chromosomes 1, 9, 15, and 16 and the Y. These regions are composed of alphoid and classical satellite DNA sequences. Using specific probes for chromosomes 1 and 16, we have performed two-color fluorescence in situ hybridization on human lymphocytes cultured in the presence of ACR. We demonstrate that for these two chromosomes (1) uncoiling and association specifically occur in classical satellite-containing regions at the first cell generation, (2) breakages also affect these regions, and (3) somatic recombinations occur between these regions and lead to translocations at the next cell generation. These results suggest that changes in methylation of repetitive DNA sequences are related to chromosomal instability occurring during cell transformation and tumorigenesis.
Descriptors		AZACYTIDINE CHROMOSOME ABERRATIONS DNA DNA, SATELLITE HETEROCHROMATIN CELL TRANSFORMATION, NEOPLASTIC CELLS, CULTURED CHROMOSOME BANDING CHROMOSOMES, HUMAN, PAIR 1 CHROMOSOMES, HUMAN, PAIR 16 DNA DAMAGE IN SITU HYBRIDIZATION, FLUORESCENCE LYMPHOCYTES METHYLATION NUCLEIC ACID DENATURATION