Author/Editor		Malovrh, Tomaž; Melkič, Enver; Kompan, Drago; Levart, Alenka; Kompan, Lidija
Title		Incorporation of conjugated linoleic acid isomers into porcine erythrocytes
Type		članek
Vol. and No.		Letnik 53, št. 3
Publication year		2014
Volume		str. 989-993
ISSN		1436-6207 - European journal of nutrition
Language		eng
Abstract		The aim of the current study was to determinethe incorporation of cis (c) 9, trans (t) 11-conjugatedlinoleic acid (CLA) and t10, c12-CLA into porcine erythrocytesboth isomers were supplemented in equalproportions. Methods The study group consisted of 16 piglets randomlyassigned into experimental and control group. Forthe period of 5 weeks, the piglets from the experimentalgroup were receiving a 1.2 % CLA supplement while thecontrols were supplemented with the same amount ofsunflower oil. For the remaining 7 weeks, the piglets werefed without a supplement. Blood samples to evaluateincorporation of CLA into erythrocyte membranes weretaken from all animals on weekly basis. Results Compared to t10, c12-CLA isomer, proportion ofc9, t11-CLA isomer in the membrane of erythrocytes washigher for the whole time of the study period. After4 weeks of feeding, it approaches the plateau. The peakvalue for both isomers was measured at the end of week 5,with a value of 3.24 g c9, t11-CLA/100 g of fatty acids anda 1.09 g t10, c12-CLA/100 g of fatty acids (p\0.0001).After cessation of supplementation, the proportion of bothisomers gradually decreased to be almost completelywashed outin 7 weeks. Conclusions During supplementation with equivalentamounts of CLA isomers, their proportion in membranes ofporcine erythrocytes increases with time, with higher proportionof c9, t11-CLA. CLA isomers probably differentlyincorporate into different cell membranes at different specieswhich could explain its various biological functions.
Keywords		linolna kislina maščobni estri metilestri eritrociti prašičev porcine erythrocytes fatty acid methyl esters conjugated linoleic acid