Author/Editor		Montpellier, Claire; Burgeois, Claire A; Kokalj-Vokač, Nadja; Muleris, Martine; Niveleau, Alain; Reynaud, Cecile; Gibaud, Anne; Malfoy, Bernard; Dutrillaux, Bernard
Title		Detection of methylcytosine-rich heterochromatin on banded chromosomes. Application to cells with various status of DNA methylation
Type		članek
Source		Cancer Genet Cytogenet
Vol. and No.		Letnik 78, št. 1
Publication year		1994
Volume		str. 87-93
Language		eng
Abstract		A procedure including incorporation of 5-bromodeoxyuridine (BrdU) in DNA and a thermal denaturation step was developed to obtain both R-banding and efficient binding of anti-5-methylcytosine antibodies on metaphase chromosomes. BrdU incorporation improved the efficiency of antibody binding disclosed by immunofluorescence staining. This method allowed semiquantitative analysis of the antibody binding sites on straightforward characterized metaphase chromosomes and was applied to normal human lymphocytes and lymphoblastoid cell lines for which DNA methylation status had been previously analyzed. A correlation was established between level of DNA methylation and the semiquantitative estimate of antibody fixation. This procedure can be used to study DNA methylation on metaphase chromosomes in transformed and cancerous cell lines.
Descriptors		CHROMOSOME BANDING CYTOSINE DNA HETEROCHROMATIN CYTOSINE DEOXYCYTIDINE FLUORESCENT ANTIBODY TECHNIQUE METHYLATION SENSITIVITY AND SPECIFICITY TUMOR CELLS, CULTURED