Author/Editor		Tsui, FW; Tsui, HW; Mok, S; Mlinarič, I; Copeland, NG; Gilbert, DJ; Jenkins, NA; Siminovitch, KA
Title		Molecular characterization and mapping of murine genes encoding three members of the stefin family of cysteine proteinase inhibitors
Type		članek
Source		Genomics
Vol. and No.		Letnik 15, št. 3
Publication year		1993
Volume		str. 507-14
Language		eng
Abstract		Stefins or Type 1 cystatins belong to a large, evolutionarily conserved protein superfamily, the members of which inhibit the papain-like cysteine proteinases. We report here on the molecular cloning and chromosomal localization of three newly identified members of the murine stefin gene family. These genes, designated herein as mouse stefins 1, 2, and 3, were isolated on the basis of their relatively increased expression in moth-eaten viable compared to normal congenic mouse bone marrow cells. The open reading frames of the stefin cDNAs encode proteins of approximately 11.5 kDa that show between 50 and 92 percent identity to sequences of stefins isolated from various other species. Data from Southern analysis suggest that the murine stefin gene family encompasses at least 6 and possibly 10-20 members, all of which appear to be clustered in the genome. Analysis of interspecific backcross mice indicates that the genes encoding the three mouse stefins all map to mouse chromosome 16, a localization that is consistent with the recent assignment of the human stefin A gene to a region of conserved homology between human chromosome 3q and the proximal region of mouse chromosome 16.
Descriptors		CHROMOSOME MAPPING CYSTATINS CYSTEINE PROTEINASE INHIBITORS MOLECULAR SEQUENCE DATA OPEN READING FRAMES SEQUENCE HOMOLOGY, AMINO ACID AMINO ACID SEQUENCE BASE SEQUENCE BONE MARROW CHROMOSOMES, HUMAN, PAIR 3 DNA GENES, REITERATED MICE MICE, INBRED C57BL MICE, MUTANT STRAINS