Author/Editor		Mason, WT; Hoyland, J; Kato, M; Akerman, S; Bunting, R; Zorec, R
Title		Dynamic video imaging of intracellular calcium and exocytosis in anterior pituitary cells secreting prolactin and growth hormone
Type		članek
Source		In: MICRO 90, London 90. IOP publishing,
Publication year		1990
Volume		str. 471-4
Language		eng
Abstract		The combination of low-light level video cameras with digital computerbased image analysis is enabling scientists to use a variety of faint fluorescent probes for study of biological activity in living cells. We have employed these techniques to study the intracellular concentration of ions including calcium and protons whilst manipulating cytosolic-based signalling pathways. Similar technology also allows us to visualise recently-released prolaction on the cell surface, using a fluorescein-conjugated prolactin antibody. This article describes some of the approaches which make possible these studies, and their application in practice to the study of secretory cells.
Descriptors		PITUITARY GLAND, ANTERIOR PROLACTIN EXOCYTOSIS RATS CATTLE IMAGE PROCESSING, COMPUTER-ASSISTED SOMATOTROPIN CALCIUM FLUORESCENT ANTIBODY TECHNIQUE MICROSCOPY, FLUORESCENCE