Author/Editor		Rumpret, Matevž; Putten, Jos; Žgur-Bertok, Darja; Starčič Erjavec, Marjanca
Title		Extra-intestinal pathogenic Escherichia coli strains with different genetic backgrounds elicit different immune responses
Type		članek
Source		In: Proceedings Ljubljana : Genetic Society of Slovenia
Publication year		2014
Volume		str. 32-38
Language		eng
Abstract		Escherichia coli (E. coli) is a normal inhabitant of the intestinal microbiota of vertebrates, serving as a barrier to colonisation by pathogens in the intestinal tract. On the other hand, some strains of E. coli are pathogenic and are important etiological agents of intestinal (intestinal pathogenic E. coli % InPEC) as well as extra-intestinal (extra-intestinal pathogenic E. coli % ExPEC) infections in humans. While commensal strains of E. coli typically harbour none or very few virulence factors, pathogenic E. coli strains usually carry multiple virulence factors, enabling them to colonise the host and cause disease. Many virulence-associated genes have been discovered in pathogenic E. coli strains. Further it is known, that there is a link between possession of virulence related genes and the phylogenetic group. To determine whether ExPEC strains belonging to different genetic backgrounds provoke different macrophage IL-6 responses, we used two different ExPEC strains, one belonging to the phylogenetic group A and the other to the phylogenetic group B2. The highest IL-6 mRNA expression levels in J774A.1 murine macrophage cells were observed upon stimulation with live bacteria. Furthermore, the B2 group strain possessing a higher virulence score was shown to elicit markedly higher macrophage IL-6 mRNA expression levels, compared to the A group strain with a lower virulence score. Our findings indicate certain differences in eliciting macrophage IL-6 response (measured by IL-6 mRNA expression levels) between pathogenic E. coli strains belonging to different phylogenetic groups, with different sets of virulence factors.
Keywords		mikrobiologija Escherichia coli imunski sistem