Author/Editor		Nowak, J; Skurzak, H; Šmilowicz, M; Kreczko, R; Ryzewska, A
Title		Surface activation markers on T and B cells in peripheral blood and in situ in patients with rheumatoid arthritis
Type		članek
Source		New Eur Rheumatol
Vol. and No.		Letnik 1, št. 2
Publication year		1993
Volume		str. 16-20
Language		eng
Abstract		The aim of the study was to analyse the appearance of activated T and B cells in lymphocytes isolated simultaneously from peripheral blood (P. B.), synovial fluid (S. F.) and synovial membrane (S. M.) of patients with rheumatoid arthritis (R. A.). The activation of lymphocytes was assessed by analysing the surface expression of DR/DP antigens and interleukin 2 receptor (II-2R) on T cells, and CDS antigen on B cells. Monoclonal antibodies with double tluorescence staining examined in a FACS (Fluorenscence Activated Cell Sorter) were used. The study was carried out on 6 patients and l2 healthy controls. The lowest proportion of activated T and B cells was found in peripheral blood. The proportion was higher in synovial tluid and highest in synovial membrane of R. A. patients. In patients operated on during R. A. remission, no activation of T and B cells could be demonstrated in P. B., while this activation was high in situ, showing that despite remission of R. A., the immune response was not suppressed locally. In addition, it was noted that the density of the CD22 antigen on the lymphocytes from P. B., S. F. and S. M. was decreased in R. A. patients as compared with P. B. lymphocytes from healthy controls, suggesting the possibility of differentiation of B cells into plasma cells. The results indicate that lymphocyte activation observed in situ during R. A. remission is not retlected in the phenotype of P. B. cells.
Descriptors		ARTHRITIS, RHEUMATOID SYNOVIAL FLUID T-LYMPHOCYTES B-LYMPHOCYTES INTERLEUKIN-2 ANTIGENS, CD ANTIBODIES, MONOCLONAL