Author/Editor		Marić, Saša; Simonović, Predrag; Razpet, Andrej
Title		Genetic characterization of broodstock brown trout from Bled fish-farm, Slovenia
Type		članek
Vol. and No.		Letnik 112, št. 2
Publication year		2010
Volume		str. 145-148
ISSN		0031-5362 - Periodicum biologorum
Language		eng
Abstract		Due to environmental and economic concerns, Bled fish-farm is interested in establishing broodstocks of native brown trout (Salmo trutta L.). Progeny would be reared and released into rivers managed by the Fishing Club Bled. In this study was performed genetic characterization of broodstock from Bled fish-farm in order to assess hybridization of native broun trout of Danubian phylogeographic lineage with trout of the allochtonous Atlantic lineage. DNA was isolated from fin clips of 20 males and 20 females from broodstock. PCR-RFLP tecnique was used for distinguishing between Atlantic and Danubian lineage on the basic of control region of mitochondrial DNA (CR mtDNA) and lactate dehydrogenase gene (LDH). Results show a hihg percentage of allochtonous genetic markers especially among females and confirm hybridization between native and introduced brown trout. Because of low percentage of native trout markers, Bled fish-farm decided to continue with collecting and genotyping fish to establish a broodstock with higher percentage of genetic markers characteristic for native trout of Danubian lineage.
Keywords		ribe postrvi Salmo trutta L. kenetska karakterizacija molekularna genetika mitohondrijska DNK Slovenija