Author/Editor		Černe, Darko
Title		Sodobna uporaba analize zunajceličnih nukleinskih kislin v laboratorijski medicini
Translated title		Contemporary use of cell-free nucleic acids analysis in laboratory medicine
Type		članek
Vol. and No.		Letnik 61, št. 3
Publication year		2010
Volume		str. 144-148
ISSN		0014-8229 - Farmacevtski vestnik
Language		slv
Abstract		Zunajcelične nukleinske kisline so nukleinske kisline, ki se nahajajo v plazmi, serumu, pa tudi v drugih bioloških vzorcih zunaj celic. Njihova analiza omogoča neinvaziven vpogled v tkivo oziroma organ, iz katerega zunajcelične nukleinske kisline izhajajo. V prispevku je predstavljena sodobna uporaba kvantitativne in kvalitativne analize zunajceličnih nukleinskih kislin v laboratorijski medicini. V prvem delu je na primeru pljučnega raka predstavljen kronološki razvoj različnih analitskih pristopov, v drugem pa uporaba na področju neinvazivne prenatalne diagnostike genskih in genomskih sprememb ploda ter bolezni oziroma zapletov v nosečnosti. V zadnjem delu prispevka so navedeni izbrani primeri uporabe na ostalih področjih laboratorijske medicine. Analiza zunajceličnih nukleinskih kislin v laboratorijski medicini pomeni sodobno raziskovalno orodje, zanimiv strokovni izziv ter alternativo oziroma dopolnilo analitiki proteinov.Cell-free nucleic acids are nucleic acids in plasma, serum or in cell-free fraction of various others biological fluids. Their analysis offers noninvasive inspection of the tissue or organ of their origin. The paper reviews contemporary use of quantitative and qualitative analysis of cellfree nucleic acids in laboratory medicine. The first part sumarises chronologic development of major analytical approaches to cell-free nucleic acids analysisin the case of medical assessment of lung cancer patients. The second part presents the use of cell-free nucleic acids analysis in noninvasive diagnosis of gene and genome alterations of the fetus and diseases and complications of pregnancy. The last part indicates the prominent examples of its use in the other areas of laboratory medicine. Cell-free nucleic acids analysis in laboratory medicine means a novel research tool, an interesting professional challenge and alternative or supplement to protein analysis.
Keywords		izvencelične nukleinske kisline analiza uporaba laboratorijska medicina