Author/Editor		Rošic, Romana; Kristl, Julijana; Baumgartner, Saša
Title		Hidrogelne obloge za vlažno celjenje ran s proteinsko učinkovino
Translated title		Protein loaded hydrogels as dressing for moist wound healing
Type		članek
Vol. and No.		Letnik 61, št. 4
Publication year		2010
Volume		str. 187-193
ISSN		0014-8229 - Farmacevtski vestnik
Language		slv
Abstract		Sodoben pristop k celjenju ran temelji na vzdrževanju vlažnega mikrookolja na rani, kar lahko dosežemo s hidrogelnimi oblogami. Raziskave so pokazale, da lahko izboljšamo celjenje kroničnih ran z vključevanjem rastnih dejavnikov ali inhibitorjev proteaz v obloge. V članku je predstavljeno oblikovanje hidrogelov iz hidroksietilceluloze ali ksantana v različnih koncentracijah in z vgrajeno modelno proteinsko učinkovino. Hidrogeli iz 3% hidroksietilceluloze ali 6% ksantana imajo ustrezne reološke lastnosti, ne glede na uporabljen medij (voda, fosfatni pufer), sterilizacijo ali način zamrzovanja pred liofilizacijo. Uporaba blagih tehnoloških pogojev izdelave omogoča ohranitev nativne strukture proteina. Za preskuse sproščanja smo razvili lastno metodo, saj uveljavljeni modeli po farmakopejah ne posnemajo stanja v kronični rani. Metoda omogoča sproščanje proteina neposredno v medij brez vmesne bariere daljše časovno obdobje. Modelni protein se je iz hidroksietilceluloznega hidrogela sproščal difuzijsko nadzorovano, kar opisuje Higuchijeva kinetika, pri čemer se je v 4 dneh sprostilo 90 % vgrajenega proteina. Takšen hidrogel zagotavlja vlažno okolje na rani, vanj pabi lahko med drugim vgradili tudi proteazni inhibitor, ki bi pomagal povrniti ravnovesje med rastnimi dejavniki in proteazami v rani, kar bi zmanjšalo vnetni proces in omogočilo hitrejše celjenje.Modern approach in the wound healing is based on maintenance of moist environment on the wound and as modern dressings hydrogels have been offered. Based on diffrent researches the healing of the wound can be improved by application of growth factors or proteasećs inhibitors. In the article the development of hydrogels from hydroxyethylcellulose or xantan in different concentrations and with incorporated model protein is presented. Hydrogels from 3% hydroxyethycellulose or 6% xantan posess appropriate rheological characteristics, regardless to the medium used (water, phosphate buffer), sterilization process or way of freezing before lyophilization. The mild technologic conditions of preparation prevent denaturation of the native structure of protein. A new method for release studies was developed, due to the fact that established pharmacopoeial models do not simulate chronic woundćs state. The method enables proteinćs release directly into the medium without barrier through longer period of time. The release of protein from hydroxyethylcellulose hydrogel is controlled by diffusion and in 4 days around90% of incorporated protein has been released. Hence, such hydrogel enables moist wound environment and incorporation of proteasećs inhibitor, which might help to regain the balance between growth factors and proteases inthe wound, what could decrease inflammation and guarantee faster healing.
Keywords		hidrogel reološke lastnosti celjenje ran modelni protein kontrolirano sproščanje