Author/Editor		Artenjak, Andrej; Locatelli, Igor; Brelih, Hana; Simonič, Darka Maša; Ulčová-Gallová, Zdenka; Swadzba, J; Musial, J; Iwaniec, T; Stojanović, Ljudmila; Conti, F.; Valesini, Guido; Avčin, Tadej; Cohen Tervaert, JW; Shoenfeld, Yehuda; Blank, Miri; Ambrožič, Aleš; Sodin-Šemrl, Snežna; Božič, Borut; Čučnik, Saša
Title		Immunoreactivity and avidity of IgG anti-ß2- glycoprotein I antibodies from patients with autoimmune diseases to different peptide clusters of ß2-glycoprotein I
Type		članek
Vol. and No.		Letnik 61, št. 1/2
Publication year		2014
Volume		str. 35-44
ISSN		1559-0755 - Immunologic research
Language		eng
Abstract		The pathogenicity of antibodies against ß2-glycoprotein I (anti-ß2GPI) depends on multiple factors such as subclass type, epitope binding and avidity. Due to their large heterogeneity, their impact on antiphospholipid syndrome (APS) on set is still not fully clarified. We studied the binding characteristics of IgG anti-ß2GPI with known avidity from sera of 201 autoimmune patients (87 with APS, 67 with APS associated with systemic lupus erythematosus (SLE), 47 with only SLE) to six ß2GPI peptides corresponding to amino acid clusters on domains I-II, II, III and III-IV by indirect ELISA and evaluated their association with clinical features of APS. Peptides A (LKTPRV;domain I-II), B (KDKATF; domain IV) and C (TLRVYK; domain III) were derived from a hexapeptide phage display library previously shown to react with pathogenic monoclonal anti-ß2GPI. Peptides D (NGPANSK; domain III), E (YNPLWFV; domain II) and F (KMDGNHP; domain III-IV) represent surface amino acid clusters on Ž2GPI. The percentage of patients positive for peptides were observed as follows: 30.3 % for peptide D, 28.90 % for B, 25.9 % for C, 24.9 %for E, 24.4 % for F and 10.0 % for A. The anti-peptide antibodies in studied serum samples were predominantly of heterogeneous avidity, followed by law avidity anti-peptide antibodies, whereas only a few were of high avidity. Positive and negative correlations were found between several anti-peptide antibodies and the rate of thrombosis. Our results indicated diverse reactivity of IgG anti-ß2GPI to different epitopes on ß2GPI. Classification of IgG anti-ß2GPI into subgroups regarding epitope specificity and avidity could represent an additional tool in understanding their pathogenicity in APS.
Keywords		antiphospholipid syndrome anti-ß2GPI antibodies ß2GPI peptides Anti-ß2GPI-related peptides antibodies avidity