Author/Editor | Zupan, S; Filipič, B; Eberl-Gregorič, E; Babič, M | |
Title | Piezoelectric immunosensors for detection of antibodies against Legionella pneumophila | |
Type | članek | |
Source | In: Abstract books of the 6th European congress on biotechnology; 1993 Jun 16 (vol 3). Firenze: European federation of biotechnology, | |
Vol. and No. | Letnik 3 | |
Publication year | 1993 | |
Volume | str. WE115-6 | |
Language | eng | |
Abstract | Different types of non-electrode biosensors were constructed to determine biologically active molecules, like antibodies. It has been repeatedly demonstrated that proteins which are firmly bound on a selective surface change the transducer's signal. Among different types of transducer the piezoelectric quartz crystals are of interest. It is of interest to detect antigen-antibody interactions performing the measurements similarly as in ELISA. Presented report describes the binding of Legionella pneumophila antigen onto the 10 MHz AT-cut piezocrystal, and subsequent direct detection of antibodies in rabbit's sera. The data from immunosensor's measurements were correlated with those from ELISA assay performed under the same conditions. | |
Descriptors | LEGIONELLA PNEUMOPHILA ANTIBODIES LEGIONNAIRES' DISEASE ANTIGENS RABBITS ANTIGEN-ANTIBODY REACTIONS CRYSTALLOGRAPHY ENZYME-LINKED IMMUNOSORBENT ASSAY TIME FACTORS SENSITIVITY AND SPECIFICITY HERPESVIRUS 1, HUMAN |