| Author/Editor | Fic, Anja; Jurković Mlakar, Simona; Juvan, Peter; Mlakar, Vid; Marc, Janja; Sollner Dolenc, Marija; Broberk, Karin; Peterlin-Mašič, Lucija | |
| Title | Genome-wide gene expression profiling of low-dose, long-term exposure of human osteosarcoma cells to bisphenol A and its analogs bisphenols AF and S | |
| Type | članek | |
| Vol. and No. | Letnik 29, št. 5 | |
| Publication year | 2015 | |
| Volume | str. 1060-1069 | |
| ISSN | 0887-2333 - Toxicology in vitro : an international journal published in association with BIBRA | |
| Language | eng | |
| Abstract | The bisphenols AF (BPAF) and S (BPS) are structural analogs of the endocrine disruptor bisphenol A (BPA), and are used in common products as a replacement for BPA. To elucidate genome-wide gene expression responses, estrogen-dependent osteosarcoma cells were cultured with 10 nM BPA, BPAF, or BPS, for 8 h and 3 months. Genome-wide gene expression was analyzed using the Illumina Expression BeadChip. Three months exposure had significant effects on gene expression, particularly for BPS, followed by BPAF and BPA, according to the number of differentially expressed genes (1980, 778, 60, respectively),the magnitude of changes in gene expression, and the number of enriched biological processes (800, 415, 33, respectively) and pathways (77, 52, 6, respectively). ŽEmbryonic skeletal system development¼ was the most enriched bone-related process, which was affected only by BPAF and BPS. Interestingly, all three bisphenols showed highest down-regulation of genes related to the cardiovascular system (e.g., NPPB, NPR3, TXNIP). BPA only and BPA/BPAF/BPS also affected genes related to the immune system and fetal development, respectively. For BPAF and BPS, the Žisoprenoid biosynthetic process¼ was enriched (up-regulated genes: HMGCS1, PDSS1, ACAT2, RCE1, DHDDS).Compared to BPA, BPAF and BPS had more effects on gene expression afterlong-term exposure. These findings stress the need for careful toxicological characterization of BPA analogs in the future. | |
| Keywords | BPA analogs BPAF BPS microarray osteosarcoma cells structural analogs |